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Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
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Chimie
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Journal JoVE Chimie
Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

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10:34 min

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February 01, 2018

DOI:

10.3791/57169-v

DOI:
10.3791/57169-v

•

10:34 min
February 01, 2018

•

7 Views
, , , , ,
1Graduate Biomedical Sciences Microbiology Theme,University of Alabama at Birmingham, 2Department of Radiology,University of Alabama at Birmingham, 3Office of the Provost,University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology,University of Alabama at Birmingham

Summary

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A protocol for the extraction of a periplasmic transition metal chaperone in the context of its native binding partners, and biophysical characterization of its substrate contents by X-ray fluorescence and radiometal uptake is presented.

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Metal UptakeGram-negative BacteriaX-ray FluorescenceRadioisotopesCell FractionationABC Transport SystemsSubstrate BindingPeriplasmOsmotic ShockSpheroplastsProtein Extraction

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Radka, C. D., Radford, L. L., Massicano, A. V., DeLucas, L. J., Lapi, S. E., Aller, S. G. Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation. J. Vis. Exp. (132), e57169, doi:10.3791/57169 (2018).

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