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שיטת Refolding יעילה וטיהור של ליגנד Chemoreceptor מחייב תחום
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Please note that all translations are automatically generated. Click here for the English version.

שיטת Refolding יעילה וטיהור של ליגנד Chemoreceptor מחייב תחום

17,408 Views

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14:25 min

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December 12, 2017

DOI:

10.3791/57092-v

DOI:
10.3791/57092-v

•

14:25 min
December 12, 2017

•

15 Views
,
1Infection and Immunity Program,Monash Biomedicine Discovery Institute, 2Department of Microbiology,Monash University, 3Biyokimya ve Moleküler Biyoloji Anabilim Dalı,Monash University

Özet

Automatically generated

הליך זה הציג את refolding של התחום מחייב ליגנד periplasmic dCACHE של קמפילובקטר jejuni chemoreceptor Tlp3 מן ההכללה וגופי הטיהור להניב כמויות מיליגרם של חלבון.

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Cite this Article

Machuca, M. A., Roujeinikova, A. Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain. J. Vis. Exp. (130), e57092, doi:10.3791/57092 (2017).

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