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Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation
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JoVE Journal Genetik
Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

9,311 Views

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09:59 min

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May 03, 2018

DOI:

10.3791/57056-v

DOI:
10.3791/57056-v

•

09:59 min
May 03, 2018

•

2 Views
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1DANDRITE – Danish Research Institute of Translational Neuroscience, Dept. of Biomedicine,Aarhus University, 2Dept. of Molecular Biology and Genetics,Aarhus University

Özet

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This method can be used to measure RNA synthesis. 5-Bromouridine is added to cells and incorporated into synthesized RNA. RNA synthesis is measured by RNA extraction immediately after labelling, followed by 5-Bromouridine-targeted immunoprecipitation of labelled RNA and analysis by reverse transcription and quantitative polymerase chain reaction.

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Cite this Article

Kofoed, R. H., Betzer, C., Lykke-Andersen, S., Molska, E., Jensen, P. H. Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation. J. Vis. Exp. (135), e57056, doi:10.3791/57056 (2018).

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