Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation
9,311 Views
•
09:59 min
•
May 03, 2018
DOI:
Summary
Automatically generated
This method can be used to measure RNA synthesis. 5-Bromouridine is added to cells and incorporated into synthesized RNA. RNA synthesis is measured by RNA extraction immediately after labelling, followed by 5-Bromouridine-targeted immunoprecipitation of labelled RNA and analysis by reverse transcription and quantitative polymerase chain reaction.
Read Article
Cite this Article
Kofoed, R. H., Betzer, C., Lykke-Andersen, S., Molska, E., Jensen, P. H. Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation. J. Vis. Exp. (135), e57056, doi:10.3791/57056 (2018).
Copy