Dergi
/
Gelişim Biyolojisi
/
Single-cell Photoconversion in Living Intact Zebrafish
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
Single-cell Photoconversion in Living Intact Zebrafish

Single-cell Photoconversion in Living Intact Zebrafish

5,851 Views

•

09:49 min

•

March 19, 2018

DOI:

10.3791/57024-v

DOI:
10.3791/57024-v

•

09:49 min
March 19, 2018

•

9 Views
,
1Department of Biological Sciences,Notre Dame Üniversitesi, 2Center for Stem Cells and Regenerative Medicine,Notre Dame Üniversitesi

Özet

Automatically generated

Here, we present a protocol to show how cell photoconversion is achieved through UV exposure to specific areas expressing the fluorescent protein, Eos, in living animals.

Explore More Videos

Single-cell PhotoconversionZebrafishFluorescent Cell PopulationsDevelopmental QuestionsSingle-cell MigrationMinimally InvasivePhotoconvertible Protein488 Nanometer Light SourceGFP Filter SetsDechorionateLow Melting Point AgaroseAnesthetized Transgenic Sox10 Eos Positive FishConfocal MicroscopyPre-conversion ImagingDorsal Root GanglionC488 Laser3D Capture

Read Article

Cite this Article

Green, L., Smith, C. J. Single-cell Photoconversion in Living Intact Zebrafish. J. Vis. Exp. (133), e57024, doi:10.3791/57024 (2018).

Download .ris file

Copy

Share Video

.

Copy