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Production and Purification of Baculovirus for Gene Therapy Application
JoVE Journal
Genetik
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JoVE Journal Genetik
Production and Purification of Baculovirus for Gene Therapy Application

Production and Purification of Baculovirus for Gene Therapy Application

DOI:
10.3791/57019-v

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06:11 min

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April 09, 2018

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, ,
1Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, 2University of Cincinnati College of Medicine, 3Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia

'Bölümler'

  • 00:04'Başlık'
  • 00:55Propagation and Harvest of Baculovirus
  • 02:20Purification of Baculovirus Vector
  • 04:00Concentration of Baculovirus
  • 05:09Results: The Yield of Infectious Baculovirus During Heparin Affinity Chromatography
  • 05:42Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

In this protocol, baculovirus is produced by transient transfection of baculovirus plasmid into Sf9 cells and amplified in a serum-free suspension culture. The supernatant is purified by heparin affinity chromatography and further concentrated by ultracentrifugation. This protocol is useful for the production and purification of baculovirus for gene therapy application.

'Etiketler'

AI-based Gene TherapyBaculovirus ProductionBaculovirus PurificationHeparin Affinity ChromatographyUltracentrifugationSf9 Cell CultureViral VectorGene TransferVaccine Development

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