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Production and Purification of Baculovirus for Gene Therapy Application
JoVE Journal
Genética
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JoVE Journal Genética
Production and Purification of Baculovirus for Gene Therapy Application

Production and Purification of Baculovirus for Gene Therapy Application

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06:11 min

April 09, 2018

DOI:

06:11 min
April 09, 2018

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Summary

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In this protocol, baculovirus is produced by transient transfection of baculovirus plasmid into Sf9 cells and amplified in a serum-free suspension culture. The supernatant is purified by heparin affinity chromatography and further concentrated by ultracentrifugation. This protocol is useful for the production and purification of baculovirus for gene therapy application.

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