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Ensayos de flujo laminar para investigar el reclutamiento leucocitario en cultivos de células vasculares y las plaquetas adherentes
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
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Ensayos de flujo laminar para investigar el reclutamiento leucocitario en cultivos de células vasculares y las plaquetas adherentes

7,432 Views

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08:50 min

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April 09, 2018

DOI:

10.3791/57009-v

DOI:
10.3791/57009-v

•

08:50 min
April 09, 2018

•

6 Views
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1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Department of Vascular Surgery, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University, School of Medicine

Özet

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Leucocitos con avidez interactúan con las células vasculares y las plaquetas después de lesión de pared del recipiente o durante la inflamación. Aquí, describimos un análisis sencillo basado en el flujo laminar para caracterizar los mecanismos moleculares que subyacen a las interacciones entre los leucocitos y sus socios celulares.

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Keyword Extraction:Leukocyte RecruitmentAdherent Vascular CellsAdherent PlateletsFlow ConditionsLaminar Flow-based AssaysİmmünolojiLeukocyte AdhesionTumor Cell AdhesionPlatelet-rich PlasmaPlatelet-poor PlasmaPlatelet BufferHydrochloric Acid EthanolRat Collagen Type OnePhosphate Buffered Saline

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Vajen, T., Heinzmann, A. C., Dickhout, A., Zhao, Z., Nagy, M., Heemskerk, J. W., Koenen, R. R. Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets. J. Vis. Exp. (134), e57009, doi:10.3791/57009 (2018).

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