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Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
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Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
Bölümler
- 00:05Başlık
- 01:15Preparation of RNAi Plates
- 02:37Preparation of C. elegans Strain BAT28
- 04:56Induction of Germ Cell Reprogramming
- 06:01Results: Assessing Reprogrammed Cells
- 06:52Conclusion
This protocol describes how to study cellular processes during cell fate conversion in Caenorhabditis elegans in vivo. Using transgenic animals, allowing heat-shock promoter-driven overexpression of the neuron fate-inducing transcription factor CHE-1 and RNAi-mediated depletion of the chromatin-regulating factor LIN-53 germ cell to neuron reprogramming can be observed in vivo.
