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rna 干扰和热休克诱导转录因子表达在重编程生殖细胞到神经元中的应用C. 线虫
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

rna 干扰和热休克诱导转录因子表达在重编程生殖细胞到神经元中的应用C. 线虫

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07:53 min

January 01, 2018

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07:53 min
January 01, 2018

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Özet

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本协议描述了如何在细胞命运转换期间研究在线虫线虫体内的细胞过程。利用转基因动物, 允许热休克启动子驱动的神经元的过度表达, 诱导转录因子 CHE-1 和 rna 介导的染色质调节因子 LIN-53 生殖细胞对神经元重新编程的损耗可以观察在体内

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