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人类胰岛神经网络的高分辨率3D 成像
JoVE Journal
Biyomühendislik
This content is Free Access.
JoVE Journal Biyomühendislik
High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network
Please note that all translations are automatically generated. Click here for the English version.

人类胰岛神经网络的高分辨率3D 成像

9,838 Views

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09:54 min

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January 29, 2018

DOI:

10.3791/56859-v

DOI:
10.3791/56859-v

•

09:54 min
January 29, 2018

•

3 Views
, , , , , , , ,
1Department of Pathology, Immunology and Experimental Medicine,Florida Üniversitesi, 2Heller School for Social Policy and Management,Brandeis University, 3Department of Medicine, College of Medicine,Florida Üniversitesi, 4Department of Biomedical Engineering, College of Engineering,Florida Üniversitesi, 5Department of Pediatrics, College of Medicine,Florida Üniversitesi

Özet

Automatically generated

在这里, 我们提出了一个协议的图像人类胰腺切片三维 (3D) 使用优化的被动清除方法。这篇手稿演示了这些程序的被动光清除后, 多免疫荧光染色, 以确定关键元素的自主神经和感觉神经网络支配人类胰岛。

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Cite this Article

Butterworth, E., Dickerson, W., Vijay, V., Weitzel, K., Cooper, J., Atkinson, E. W., Coleman, J. E., Otto, K. J., Campbell-Thompson, M. High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network. J. Vis. Exp. (131), e56859, doi:10.3791/56859 (2018).

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