A Novel <em>In Vitro</em> Wound Healing Assay to Evaluate Cell Migration

Floriana Cappiello; Bruno Casciaro; Maria Luisa Mangoni

doi:10.3791/56825

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A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

DOI:

10.3791/56825-v

•

08:55 min

•

March 17, 2018

•

Floriana Cappiello, Bruno Casciaro, Maria Luisa Mangoni

¹Department of Biochemical Sciences,Sapienza University of Rome

Bölümler

00:00Başlık
01:28Cell Preparation
03:34Cell Seeding in the Culture Inserts
04:30Pseudo-wound Healing Assay
05:52Image Analysis
06:53Representative Results
07:34Effects of Inhibitors on Cells Migration
08:33Conclusions

Özet

Otomatik Çeviri

Here, we present a protocol to evaluate the effect of peptides on the migration of bronchial epithelial cells. This method allows for the rapid and highly reproducible obtainment of quantitative data on the speed of cell migration and wound closure.

Etiketler

Cell Migration Wound Healing In Vitro Assay Immunomodulatory Molecules Antimicropeptides Cell Monolayer Pseudo-wound Image Analysis Bronchial Epithelial Cells Epidermal Growth Factor Receptor

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