Journal
/
Immunologie und Infektion
/
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
JoVE Journal
Immunologie und Infektion
Author Produced
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

DOI:
10.3791/56825-v

•

08:55 min

•

March 17, 2018

•

, ,
1Department of Biochemical Sciences,Sapienza University of Rome

Kapitel

  • 00:00Titel
  • 01:28Cell Preparation
  • 03:34Cell Seeding in the Culture Inserts
  • 04:30Pseudo-wound Healing Assay
  • 05:52Image Analysis
  • 06:53Representative Results
  • 07:34Effects of Inhibitors on Cells Migration
  • 08:33Conclusions

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present a protocol to evaluate the effect of peptides on the migration of bronchial epithelial cells. This method allows for the rapid and highly reproducible obtainment of quantitative data on the speed of cell migration and wound closure.

Tags

Cell MigrationWound HealingIn Vitro AssayImmunomodulatory MoleculesAntimicropeptidesCell MonolayerPseudo-woundImage AnalysisBronchial Epithelial CellsEpidermal Growth Factor Receptor

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

Read Article