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Une préparation optimale du formol fixé des échantillons à base de peptides Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
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January 16, 2018
DOI:
1Mass Spectrometry Core Facility,University of Sydney, 2Proteomics Core Facility,University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney
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Ce protocole décrit une méthode fiable et reproductible pour la préparation axée sur la sublimation du formol fixé tissu destiné à la spectrométrie de masse d’imagerie.
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O'Rourke, M. B., Padula, M. P., Smith, C., Youssef, P., Cordwell, S., Witting, P., Sutherland, G., Crossett, B. Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows. J. Vis. Exp. (131), e56778, doi:10.3791/56778 (2018).
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