Dergi
/
Biyokimya
/
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

DOI:
10.3791/56778-v

•

08:16 min

•

January 16, 2018

•

, , , , , , ,
1Mass Spectrometry Core Facility,University of Sydney, 2Proteomics Core Facility,University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney

Bölümler

  • 00:05Başlık
  • 00:29Preparation of Reagents and Equipment
  • 01:21Preparation of Tissue Sections and Methylene Crosslink Hydrolysis
  • 02:40Tissue Digestion
  • 03:57Matrix Coating via Sublimation
  • 05:26Recrystallization and Instrumentation
  • 06:20Results: Correctly and Incorrectly Prepared Human Brain Tissue
  • 07:07Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol describes a reproducible and reliable method for the sublimation-based preparation of formalin fixed tissue destined for imaging mass spectrometry.

Etiketler

Formalin FixedPeptide Mass Spectrometry ImagingNitrocellulose CoatingVapor ChamberXyleneEthanolCarnoy’s FluidTris BufferPressure CookerTrypsin

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Read Article