需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
7,889 Views
•
08:16 min
•
January 16, 2018
DOI:
1Mass Spectrometry Core Facility,University of Sydney, 2Proteomics Core Facility,University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney
Summary
Automatically generated
This protocol describes a reproducible and reliable method for the sublimation-based preparation of formalin fixed tissue destined for imaging mass spectrometry.
Read Article
Cite this Article
O'Rourke, M. B., Padula, M. P., Smith, C., Youssef, P., Cordwell, S., Witting, P., Sutherland, G., Crossett, B. Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows. J. Vis. Exp. (131), e56778, doi:10.3791/56778 (2018).
Copy