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Retrograde neuroanatomische tracering van Phrenic motorische neuronen in muizen
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JoVE Journal Nörobilim
Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice
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Retrograde neuroanatomische tracering van Phrenic motorische neuronen in muizen

12,296 Views

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09:05 min

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February 22, 2018

DOI:

10.3791/56758-v

DOI:
10.3791/56758-v

•

09:05 min
February 22, 2018

•

11 Views
, , , ,
1URVI, NARILIS,Université de Namur, 2URPhyM, NARILIS,Université de Namur

Özet

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Hier beschrijven we een protocol voor het identificeren van phrenic motorische neuronen in muizen nadat pleurale levering van fluorophore geconjugeerd cholera-toxine subeenheid bèta. Twee technieken worden vergeleken om te injecteren de pleurale Holte: transdiaphragmatic versus transthoracic benaderingen.

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Vandeweerd, J., Hontoir, F., De Knoop, A., De Swert, K., Nicaise, C. Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice. J. Vis. Exp. (132), e56758, doi:10.3791/56758 (2018).

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