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Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection
JoVE Journal
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JoVE Journal Nörobilim
Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

DOI:
10.3791/56757-v

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09:23 min

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March 09, 2018

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, ,
1Department of Anatomy, Chicago College of Osteopathic Medicine (CCOM),Midwestern University, 2Department of Microbiology and Immunology, Chicago College of Osteopathic Medicine (CCOM),Midwestern University

Bölümler

  • 00:04Başlık
  • 00:55Harvesting DRG from Mouse Embryos
  • 03:59Isolating Single Neurons from DRG and HSV-1 Infection
  • 06:07Immunofluorescence
  • 07:11Results: Investigation of HSV-1 Entry Model in DRG-derived Dissociated Cells
  • 08:06Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

In this report, the advantages of organotypic cultures and dissociated primary cultures of mouse-derived dorsal root ganglia are highlighted to investigate a wide range of mechanisms associated with neuron-glial interaction, neuroplasticity, neuroinflammation, and response to viral infection.

Etiketler

Dorsal Root GangliaDRGExplantDissociated Cell ModelNeuroplasticityNeuroinflammationViral InfectionSpinal CordPeripheral NerveIntervertebral ForamenPrimary Cell Culture

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