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Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection
JoVE Journal
神経科学
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JoVE Journal 神経科学
Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

DOI:

09:23 min

March 09, 2018

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  • 00:04標題
  • 00:55Harvesting DRG from Mouse Embryos
  • 03:59Isolating Single Neurons from DRG and HSV-1 Infection
  • 06:07Immunofluorescence
  • 07:11Results: Investigation of HSV-1 Entry Model in DRG-derived Dissociated Cells
  • 08:06Conclusion

概要

自動翻訳

In this report, the advantages of organotypic cultures and dissociated primary cultures of mouse-derived dorsal root ganglia are highlighted to investigate a wide range of mechanisms associated with neuron-glial interaction, neuroplasticity, neuroinflammation, and response to viral infection.

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