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An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration
1Research Center for Translational Medicine,Tongji University School of Medicine, 2Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital,Tongji University School of Medicine, 3Department of Anatomy and Neurobiology,Tongji University School of Medicine, 4Shanghai Advanced Research Institute, University of Chinese Academy of Sciences,Chinese Academy of Sciences, 5Sino-Danish College, University of Chinese Academy of Sciences,Chinese Academy of Sciences
Bölümler
- 00:05Başlık
- 01:14Fly Collection
- 02:13Automated RING Assay
- 03:56RING Assay Data Analysis
- 05:19Results: Adult Climbing Behavior in Drosophila melanogaster
- 06:03Conclusion
This step-by-step protocol analyzes Drosophila negative geotaxis behavior using an automated multi-cylinder system that hosts hundreds of flies and synchronizes their action by an electric motor. Upon synchronization, fly negative geotaxis behavior is assayed, digitally recorded, and analyzed using the self-designed RflyDetection software.
