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An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration
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September 12, 2017
DOI:
1Research Center for Translational Medicine,Tongji University School of Medicine, 2Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital,Tongji University School of Medicine, 3Department of Anatomy and Neurobiology,Tongji University School of Medicine, 4Shanghai Advanced Research Institute, University of Chinese Academy of Sciences,Chinese Academy of Sciences, 5Sino-Danish College, University of Chinese Academy of Sciences,Chinese Academy of Sciences
Summary
Automatically generated
This step-by-step protocol analyzes Drosophila negative geotaxis behavior using an automated multi-cylinder system that hosts hundreds of flies and synchronizes their action by an electric motor. Upon synchronization, fly negative geotaxis behavior is assayed, digitally recorded, and analyzed using the self-designed RflyDetection software.
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Cite this Article
Cao, W., Song, L., Cheng, J., Yi, N., Cai, L., Huang, F., Ho, M. An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration. J. Vis. Exp. (127), e56507, doi:10.3791/56507 (2017).
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