This protocol describes a novel three-dimensional in vitro model, where corneal stromal cells and differentiated neuronal cells are cultured together to assist in the examination and understanding of the interactions of the two cell types.
Sharif, R., Priyadarsini, S., Rowsey, T. G., Ma, J., Karamichos, D. Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea. J. Vis. Exp. (131), e56308, doi:10.3791/56308 (2018).