Journal
/
发育生物学
/
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

DOI:
10.3791/56308-v

•

07:35 min

•

January 24, 2018

•

, , , ,
1Department of Cell Biology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology/Dean McGee Eye Institute,University of Oklahoma Health Sciences Center, 3Department of Physiology, Harold Hamm Diabetes Center,University of Oklahoma Health Sciences Center

Chapters

  • 00:05Title
  • 01:17Isolation of Primary Cells
  • 04:04Primary HCF Culture and 3D Construct Assembly
  • 05:00Cell Differentiation and Co-culture
  • 06:17Results: Transmission Electron Microscopy of the 3D Self-assembled Construct
  • 06:48Conclusion

Summary

自动翻译

自动翻译

This protocol describes a novel three-dimensional in vitro model, where corneal stromal cells and differentiated neuronal cells are cultured together to assist in the examination and understanding of the interactions of the two cell types.

Tags

Stromal-nerve InteractionsHuman CorneaCorneal DiseasesCorneal DystrophiesExtracellular MatrixCell-cell InteractionsDrug ScreeningCorneal Nerve DamageCorneal Nerve Regeneration

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/56308?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/cn/56308?article_type=v

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
check_url/cn/56308?article_type=v

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
check_url/cn/56308?article_type=v

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
check_url/cn/56308?article_type=v

Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
check_url/cn/56308?article_type=v

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
check_url/cn/56308?article_type=v

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
check_url/cn/56308?article_type=v

Live Imaging of Mouse Secondary Palate Fusion
check_url/cn/56308?article_type=v

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
check_url/cn/56308?article_type=v

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Read Article