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Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
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January 31, 2018
DOI:
1Department of Biological Chemistry, School of Medicine,Kaliforniya Üniversitesi, Irvine, 2Beckman Laser Institute and Medical Clinic,Kaliforniya Üniversitesi, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences,Kaliforniya Üniversitesi, Irvine, 4Department of Biomedical Engineering and Surgery,Kaliforniya Üniversitesi, Irvine
Özet
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The goal of this protocol is to describe how to use laser microirradiation to induce different types of DNA damage, including relatively simple strand breaks and complex damage, to study DNA damage signaling and repair factor assembly at damage sites in vivo.
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Kong, X., Cruz, G. M., Silva, B. A., Wakida, N. M., Khatibzadeh, N., Berns, M. W., Yokomori, K. Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage. J. Vis. Exp. (131), e56213, doi:10.3791/56213 (2018).
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