Journal
/
Genética
/
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

DOI:
10.3791/56213-v

•

10:44 min

•

January 31, 2018

•

, , , , , ,
1Department of Biological Chemistry, School of Medicine,University of California, Irvine, 2Beckman Laser Institute and Medical Clinic,University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences,University of California, Irvine, 4Department of Biomedical Engineering and Surgery,University of California, Irvine

Capítulos

  • 00:05Título
  • 01:25Transient Transfection
  • 04:09Titration of Laser Input Power
  • 05:40Quantitative Fluorescence Analysis of Immunostained or Live Cells
  • 06:54Cell Cycle and G1 Phase Synchronization
  • 07:46Results: In Vivo Cellular Responses to Complex DNA Damage
  • 09:27Conclusion

Summary

Tadução automática

Tadução automática

The goal of this protocol is to describe how to use laser microirradiation to induce different types of DNA damage, including relatively simple strand breaks and complex damage, to study DNA damage signaling and repair factor assembly at damage sites in vivo.

Tags

Laser MicroirradiationDNA DamageDNA RepairDNA Repair FactorsCellular ResponseReal-time AnalysisSingle-cell AnalysisFluorescently-tagged Proteins53BP1NTH1Liposome-mediated Transfection

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

The Nematode Caenorhabditis Elegans – A Versatile In Vivo Model to Study Host-microbe Interactions

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Simultaneous Video-EEG-ECG Monitoring to Identify Neurocardiac Dysfunction in Mouse Models of Epilepsy

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

Read Article