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Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing
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Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing
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September 01, 2017
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The goal of this protocol is to alter the penetrance of lethal skeletal mutant phenotypes in zebrafish by selective breeding. Lethal mutants cannot be grown to adulthood and bred themselves, therefore this protocol describes a method for tracking and selecting penetrance through multiple generations by progeny testing.
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Brooks, E. P., Nichols, J. T. Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing. J. Vis. Exp. (127), e56200, doi:10.3791/56200 (2017).
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