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Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing
Chapters
- 00:05Title
- 01:05KASP Genotyping
- 03:18Pairwise Cross Sibling Heterozygous and Embryo Rearing
- 04:13Alcian Blue/Alizarin Red Cartilage and Bone Staining
- 05:42Phenotype Scoring and Family Selection
- 06:49Results: Selective Breeding for Skeletal Penetrance in Zebrafish
- 07:58Conclusion
The goal of this protocol is to alter the penetrance of lethal skeletal mutant phenotypes in zebrafish by selective breeding. Lethal mutants cannot be grown to adulthood and bred themselves, therefore this protocol describes a method for tracking and selecting penetrance through multiple generations by progeny testing.
