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Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
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Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

7,861 Views

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10:12 min

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August 09, 2017

DOI:

10.3791/56063-v

DOI:
10.3791/56063-v

•

10:12 min
August 09, 2017

•

1 Views
, , , ,
1Brain Institute,Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering,ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience,Johns Hopkins Üniversitesi

Özet

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Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.

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Cite this Article

Landeira, B. S., Araújo, J. A. d. M., Schroeder, T., Müller, U., Costa, M. R. Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System. J. Vis. Exp. (126), e56063, doi:10.3791/56063 (2017).

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