ジャーナル
/
神経科学
/
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

7,867 Views

•

10:12 min

•

August 09, 2017

DOI:

10.3791/56063-v

DOI:
10.3791/56063-v

•

10:12 min
August 09, 2017

•

1 Views
, , , ,
1Brain Institute,Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering,ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University

概要

Automatically generated

Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.

Explore More Videos

Live ImagingPrimary Cerebral Cortex Cells2D Culture SystemNeural Stem CellsNeuronsGlial CellsNeurodevelopmentCell LineageCell DivisionCell CycleCell GrowthEmbryonic BrainDorsolateral TelencephalonCell DissociationTrypsin-EDTAProliferation MediumPoly-D-lysine

Read Article

Cite this Article

Landeira, B. S., Araújo, J. A. d. M., Schroeder, T., Müller, U., Costa, M. R. Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System. J. Vis. Exp. (126), e56063, doi:10.3791/56063 (2017).

Download .ris file

Copy

Share Video

.

Copy