This study presents a technique for the isolation of neurons from WT neonatal mice. It requires the careful dissection of the spinal cord from the neonatal mouse, followed by the separation of neurons from the spinal cord tissue through mechanical and enzymatic cleavage.
Eldeiry, M., Yamanaka, K., Reece, T. B., Aftab, M. Spinal Cord Neurons Isolation and Culture from Neonatal Mice. J. Vis. Exp. (125), e55856, doi:10.3791/55856 (2017).