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Spinal Cord Neurons Isolation and Culture from Neonatal Mice
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Spinal Cord Neurons Isolation and Culture from Neonatal Mice

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

DOI:

07:49 min

July 11, 2017

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Kapitel

  • 00:05Titel
  • 00:58Harvesting Spinal Cords
  • 02:27Isolating Neurons
  • 05:53Results: Immunofluorescent Staining of Neurons Using Neuronal Cytoskeletal Marker – MAP2
  • 06:47Conclusion

Summary

Automatische Übersetzung

This study presents a technique for the isolation of neurons from WT neonatal mice. It requires the careful dissection of the spinal cord from the neonatal mouse, followed by the separation of neurons from the spinal cord tissue through mechanical and enzymatic cleavage.

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