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Hypoxische preconditioning van merg-afgeleide progenitorcellen als bron voor de generatie van volwassen Schwann-cellen
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
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Hypoxische preconditioning van merg-afgeleide progenitorcellen als bron voor de generatie van volwassen Schwann-cellen

7,179 Views

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10:16 min

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June 14, 2017

DOI:

10.3791/55794-v

DOI:
10.3791/55794-v

•

10:16 min
June 14, 2017

•

2 Views
, , , ,
1School of Biomedical Sciences,The University of Hong Kong, 2Department of Orthopaedics and Traumatology,The University of Hong Kong

Özet

Automatically generated

Spiercellen (MSC's) met neurale potentieel bestaan ​​in het beenmerg. Ons protocol verrijkt deze populatie van cellen via hypoxische preconditioning en wijst hen vervolgens op om volwassen Schwann cellen te worden.

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Hypoxic PreconditioningMarrow-derived Progenitor CellsSchwann CellsStem CellRegenerative MedicineNeural ProgenitorsBone Marrow Stromal CellsAutologous Cell TherapyMSC Growth MediumCell DissociationHypoxia ChamberHepesNitrogenOxygen

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Tsui, Y., Mung, A. K., Chan, Y., Shum, D. K., Shea, G. K. Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells. J. Vis. Exp. (124), e55794, doi:10.3791/55794 (2017).

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