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Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells

Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells

DOI:
10.3791/55794-v

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10:16 min

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June 14, 2017

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, , , ,
1School of Biomedical Sciences,The University of Hong Kong, 2Department of Orthopaedics and Traumatology,The University of Hong Kong

Capitoli

  • 00:05Titolo
  • 00:47MSC Culture Establishment and Expansion
  • 02:13Schwann Cell-like Cell (SCLC) Generation
  • 04:40Dorsal Root Ganglion (DRG) Purification and SCLC Co-culture
  • 07:44Results: Representative Schwann Cell Generation
  • 09:08Conclusion

Summary

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Marrow stromal cells (MSCs) with neural potential exist within the bone marrow. Our protocol enriches this population of cells via hypoxic preconditioning and thereafter directs them to become mature Schwann cells.

Tags

Hypoxic PreconditioningMarrow-derived Progenitor CellsSchwann CellsStem CellRegenerative MedicineNeural ProgenitorsBone Marrow Stromal CellsAutologous Cell TherapyMSC Growth MediumCell DissociationHypoxia ChamberHepesNitrogenOxygen

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