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Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
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Biologie du développement
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Journal JoVE Biologie du développement
Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells

Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells

7,179 Views

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10:16 min

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June 14, 2017

DOI:

10.3791/55794-v

DOI:
10.3791/55794-v

•

10:16 min
June 14, 2017

•

2 Views
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1School of Biomedical Sciences,The University of Hong Kong, 2Department of Orthopaedics and Traumatology,The University of Hong Kong

Summary

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Marrow stromal cells (MSCs) with neural potential exist within the bone marrow. Our protocol enriches this population of cells via hypoxic preconditioning and thereafter directs them to become mature Schwann cells.

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Hypoxic PreconditioningMarrow-derived Progenitor CellsSchwann CellsStem CellRegenerative MedicineNeural ProgenitorsBone Marrow Stromal CellsAutologous Cell TherapyMSC Growth MediumCell DissociationHypoxia ChamberHepesNitrogenOxygen

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Tsui, Y., Mung, A. K., Chan, Y., Shum, D. K., Shea, G. K. Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells. J. Vis. Exp. (124), e55794, doi:10.3791/55794 (2017).

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