This content is Free Access.
Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
11,820 Views
•
14:02 min
•
April 21, 2017
DOI:
Özet
Automatically generated
This paper describes a methodology to prepare cardiovascular tissue samples for MS analysis that allows for (1) the analysis of ECM protein composition, (2) the identification of glycosylation sites, and (3) the compositional characterization of glycan forms. This methodology can be applied, with minor modifications, to the study of the ECM in other tissues.
Read Article
Cite this Article
Barallobre-Barreiro, J., Baig, F., Fava, M., Yin, X., Mayr, M. Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry. J. Vis. Exp. (122), e55674, doi:10.3791/55674 (2017).
Copy