This content is Free Access.
Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
章
- 00:05標題
- 01:05Extraction of Extracellular Matrix Proteins
- 03:52Protein Precipitation
- 04:57Sequential Deglycosylation for the Assessment of N-glycosylation Site Occupancy
- 06:05Peptide Cleanup Using C18 Column
- 08:32Glycopeptide Enrichment
- 11:32Results: Glycopeptide Extraction and Analysis
- 13:18Conclusion
This paper describes a methodology to prepare cardiovascular tissue samples for MS analysis that allows for (1) the analysis of ECM protein composition, (2) the identification of glycosylation sites, and (3) the compositional characterization of glycan forms. This methodology can be applied, with minor modifications, to the study of the ECM in other tissues.
