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Determinazione di alta affinità antigene-anticorpo cinetica del legame con quattro piattaforme biosensore
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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Determinazione di alta affinità antigene-anticorpo cinetica del legame con quattro piattaforme biosensore

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15:27 min

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April 17, 2017

DOI:

10.3791/55659-v

DOI:
10.3791/55659-v

•

15:27 min
April 17, 2017

•

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1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery,Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science,Columbia University

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Descriviamo qui Protocolli per la misurazione di anticorpo-antigene affinità e cinetica usando quattro piattaforme biosensore comunemente utilizzati vincolante.

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Antibody-antigen BindingBiosensor PlatformsKinetic DataDrug DiscoveryBiacore T100ProteOn XPR36Experimental DesignKinetic MeasurementsHigh-affinity InteractionsData ConsistencyOperational Efficiency

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Yang, D., Singh, A., Wu, H., Kroe-Barrett, R. Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms. J. Vis. Exp. (122), e55659, doi:10.3791/55659 (2017).

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