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Détermination de l'anticorps-antigène à haute affinité de liaison Kinetics En utilisant quatre plates-formes biocapteurs
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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Détermination de l'anticorps-antigène à haute affinité de liaison Kinetics En utilisant quatre plates-formes biocapteurs

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15:27 min

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April 17, 2017

DOI:

10.3791/55659-v

DOI:
10.3791/55659-v

•

15:27 min
April 17, 2017

•

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1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery,Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science,Columbia University

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Nous décrivons ici les protocoles pour la mesure de l'affinité de liaison anticorps-antigène et la cinétique en utilisant quatre plates-formes de biocapteurs couramment utilisés.

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Yang, D., Singh, A., Wu, H., Kroe-Barrett, R. Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms. J. Vis. Exp. (122), e55659, doi:10.3791/55659 (2017).

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