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高亲和力抗体 - 抗原的结合动力学的测定，使用四个生物传感器平台
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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高亲和力抗体 - 抗原的结合动力学的测定，使用四个生物传感器平台

20,107 Views

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15:27 min

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April 17, 2017

DOI:

10.3791/55659-v

DOI:
10.3791/55659-v

•

15:27 min
April 17, 2017

•

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1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery,Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science,Columbia University

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在这里，我们描述了抗原抗体的结合亲和力和使用四种常用的生物传感器平台动力学测量协议。

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Yang, D., Singh, A., Wu, H., Kroe-Barrett, R. Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms. J. Vis. Exp. (122), e55659, doi:10.3791/55659 (2017).

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