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体外和活体评估移植受体的 T、B 和髓细胞抑制活性和体液反应
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
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体外和活体评估移植受体的 T、B 和髓细胞抑制活性和体液反应

DOI:
10.3791/55510-v

•

18:48 min

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August 12, 2017

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, ,
1Centre de Recherche en Transplantation et Immunologie UMR 1064,INSERM, Université de Nantes, 2Institut de Transplantation Urologie Néphrologie (ITUN),CHU Nantes

Bölümler

  • 00:00Başlık
  • 00:37LEW.1W to LEW.1A Allograft Procedure
  • 02:18Isolation of Allograft APCs (pDCs)
  • 06:12Isolation of Responder Effector T Cells and Regulatory T Cells (CD4+ and CD8+CD45RClow)
  • 10:30CFSE Labeling of Responder Cells to Measure their Proliferation
  • 12:09FACS Staining and Analysis After 6 Days of Coculture
  • 13:49In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
  • 16:33Representative Results
  • 18:03Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在这里, 我们提出了一个诱导耐受性的移植, 并评估体外和体内的抑制能力的不同细胞亚群从接受者和免疫状态的受体向施主或外源性抗原。

Etiketler

TransplantToleranceRegulatory CellsSuppressive CapacityHumoral ResponseIn VitroIn VivoSplenocytesLewis RatCollagenaseEDTACell Isolation

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