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JoVE Journal Immunology and Infection
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
 

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Article DOI: 10.3791/55510-v • 18:49 min • August 12th, 2017
August 12th, 2017 •
1,2, 1,2, 1,2
1Centre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, 2Institut de Transplantation Urologie Néphrologie (ITUN), CHU Nantes

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Here, we present a protocol to induce tolerance in transplantation, and assess in vitro and in vivo the suppressive capacity of distinct cell subsets from the recipient and the immune status of the recipient toward donor or exogenous antigens.

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In Vitro In Vivo Assessment T Cells B Cells Myeloid Cells Suppressive Activity Humoral Responses Transplant Recipients Tolerance Induction Regulatory Cell Population Immune Response Exogenous Antigen Protocol In Vitro Assessment In Vivo Assessment Donor Lewis 1W Rat Isoflurane O2 Inhalation N20 1% Dorsal Decubitus Thoracotomy Vena Cava Ligatures Pulmonary Artery Aorta Cold NaCl 0.9% Recipient Rat Lewis 1A Rat Eight To 12 Weeks Cephalo-pubic Laparotomy. Externalize Intestines Abdominal Blood Vessels Terminal Lateral Anastomosis Graphed Aorta Abdominal Aorta Clamps Suture Muscular Plane And Skin Disinfect With Betadine Meloxicam Subcutaneously Terramycin And Buprenorphine Intramuscularly
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