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Generation of Fluorescent Protein Fusions in Candida Species
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Genetik
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JoVE Journal Genetik
Generation of Fluorescent Protein Fusions in Candida Species

Generation of Fluorescent Protein Fusions in Candida Species

DOI:
10.3791/55333-v

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09:27 min

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March 04, 2017

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, ,
1Pediatri Anabilim Dalı,University of Minnesota, 2Department of Molecular Microbiology and Biotechnology,Tel Aviv Üniversitesi

Bölümler

  • 00:05Başlık
  • 01:20Generation of Fluorescent Protein Cassettes by PCR
  • 03:26Transform Candida Cells with Fluorescent Protein DNA Cassette
  • 07:31Results: Generation of GFP and mCherry Fusions to Eno1
  • 08:46Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

PCR-mediated gene modification can be used to generate fluorescent protein fusions in Candida species, which facilitates visualization and quantitation of yeast cells and proteins. Herein, we present a strategy for constructing a fluorescent protein fusion (Eno1-FP) in Candida parapsilosis.

Etiketler

Fluorescent Protein FusionCandida SpeciesPCR-mediated Gene ModificationFluorescently Tagged Candida StrainsIn Vitro And In Vivo AnalysesCandida AlbicansCandida ParapsilosisPCR Master MixAgarose Gel ElectrophoresisDNA PrecipitationDNA Resuspension

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