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Generation of Fluorescent Protein Fusions in Candida Species
JoVE Revista
Genética
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JoVE Revista Genética
Generation of Fluorescent Protein Fusions in Candida Species

Generation of Fluorescent Protein Fusions in Candida Species

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09:27 min

March 04, 2017

DOI:

09:27 min
March 04, 2017

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Summary

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PCR-mediated gene modification can be used to generate fluorescent protein fusions in Candida species, which facilitates visualization and quantitation of yeast cells and proteins. Herein, we present a strategy for constructing a fluorescent protein fusion (Eno1-FP) in Candida parapsilosis.

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