Dergi
/
Genetik
/
Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
JoVE Journal
Genetik
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Genetik
Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

7,198 Views

•

08:14 min

•

February 25, 2017

DOI:

10.3791/55312-v

DOI:
10.3791/55312-v

•

08:14 min
February 25, 2017

•

4 Views
,
1Department of Biological Chemistry and Molecular Pharmacology,Harvard Tıp Fakültesi

Özet

Automatically generated

We describe sensitive, gel-based discontinuous assays to examine the kinetics of lagging-strand initiation using the replication proteins of bacteriophage T7.

Explore More Videos

DNA SynthesisLagging-strandBacteriophage T7DNA ReplicationDNA PrimaseDNA PolymeraseOkazaki Fragment SynthesisRadioactive MaterialsFormamide Dye BufferGene Four ProteinGP Four Primase HelicaseMagnesium ChlorideDenaturing Gel ElectrophoresisRapid Quench Flow Instrument

Read Article

Cite this Article

Hernandez, A. J., Richardson, C. C. Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins. J. Vis. Exp. (120), e55312, doi:10.3791/55312 (2017).

Download .ris file

Copy

Share Video

.

Copy