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Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
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JoVE Journal Genetica
Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

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08:14 min

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February 25, 2017

DOI:

10.3791/55312-v

DOI:
10.3791/55312-v

•

08:14 min
February 25, 2017

•

4 Views
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1Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Summary

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We describe sensitive, gel-based discontinuous assays to examine the kinetics of lagging-strand initiation using the replication proteins of bacteriophage T7.

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Hernandez, A. J., Richardson, C. C. Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins. J. Vis. Exp. (120), e55312, doi:10.3791/55312 (2017).

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