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Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
JoVE Journal
Genetik
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JoVE Journal Genetik
Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

DOI:
10.3791/55267-v

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09:37 min

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March 05, 2017

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, , ,
1Developmental Biology Program,Sloan Kettering Institute

Bölümler

  • 00:05Başlık
  • 00:52Generation of iCas9 hPSC Lines
  • 04:15gRNA Transfection of iCas9 hPSCs
  • 06:00In Vitro hPSC Differentiation into Glucose-responsive Pancreatic β Cells
  • 07:48Results: Efficient Genetic Modification in hPSCs Using the iCRISPR Platform
  • 08:50Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Protocols to generate hPSC mutant lines using the iCRISPR platform and to differentiate hPSCs into glucose-responsive β-like cells are described. Combining genome editing technology with hPSC-directed differentiation provides a powerful platform for the systematic analysis of the role of lineage determinants in human development and disease progression.

Etiketler

Genome EditingDirected DifferentiationHPSCsPancreatic DevelopmentICRISPRVitronectinROCK InhibitorElectroporationCell CultureCell SuspensionCell CountPlasmidsGene Modification

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