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Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
Chapters
- 00:05Title
- 00:52Generation of iCas9 hPSC Lines
- 04:15gRNA Transfection of iCas9 hPSCs
- 06:00In Vitro hPSC Differentiation into Glucose-responsive Pancreatic β Cells
- 07:48Results: Efficient Genetic Modification in hPSCs Using the iCRISPR Platform
- 08:50Conclusion
Protocols to generate hPSC mutant lines using the iCRISPR platform and to differentiate hPSCs into glucose-responsive β-like cells are described. Combining genome editing technology with hPSC-directed differentiation provides a powerful platform for the systematic analysis of the role of lineage determinants in human development and disease progression.
