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Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents
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January 22, 2017
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Here, we present a protocol for hydraulic extrusion of the spinal cord as well as identification and isolation of specific dorsal root ganglia (DRGs) in the same rodent. Compared to standard spinal cord isolation methods, this method is significantly faster and reduces the risk of tissue damage.
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Richner, M., Jager, S. B., Siupka, P., Vaegter, C. B. Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents. J. Vis. Exp. (119), e55226, doi:10.3791/55226 (2017).
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