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Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents
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Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents
Kapitel
- 00:00Titel
- 02:04Preparation of Syringe for Hydraulic Extrusion of Spinal Cord
- 02:24Euthanasia
- 02:40Isolation of the Spinal Column
- 03:23Hydraulic Extrusion of the Spinal Cord
- 04:02Identification of Dorsal Root Ganglia (DRGs)
- 04:56Isolation of DRGs
- 05:50Processing of Tissues for Further Analysis
- 07:24Representative Results
- 07:37Conclusion
Here, we present a protocol for hydraulic extrusion of the spinal cord as well as identification and isolation of specific dorsal root ganglia (DRGs) in the same rodent. Compared to standard spinal cord isolation methods, this method is significantly faster and reduces the risk of tissue damage.
