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A base Fluorescence-lymphocytaire Assay Convient pour Criblage à haut débit des petites molécules
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JoVE Journal İmmünoloji ve Enfeksiyon
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
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A base Fluorescence-lymphocytaire Assay Convient pour Criblage à haut débit des petites molécules

10,182 Views

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08:43 min

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March 10, 2017

DOI:

10.3791/55199-v

DOI:
10.3791/55199-v

•

08:43 min
March 10, 2017

•

4 Views
, , , ,
1Department of Microbiology, Infectiology and Immunology,Université de Montréal, 2Department of Pharmacology and Physiology,Université de Montréal, 3Molecular Biology Program,Université de Montréal

Özet

Automatically generated

Nous présentons dans l'étude d'un nouveau test basé sur la fluorescence en utilisant des lymphocytes dérivés d'une souris transgénique. Ce dosage est adapté pour le criblage à haut débit (HTS) de petites molécules dotées de la capacité de l'une ou l'inhibition de la promotion de l'activation des lymphocytes.

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Cite this Article

Fouda, A., Tahsini, M., Khodayarian, F., Al-nafisah, F., Rafei, M. A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules. J. Vis. Exp. (121), e55199, doi:10.3791/55199 (2017).

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